Phospho mtor ser2448

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August undefined, 2024

Webthe complex. Phosphorylation of mTOR at Ser2448 is carried out directly by AKT kinase as well as p70S6 kinase acting as a feedback signal. Phosphorylation at this site is a biomarker for the activation state of the PI-3 kinase pathway as well as the activation status of mTOR. Activation of mTOR leads to phosphorylation of PRAS40, WebA synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR. Formulation. 1X PBS, 0.09% NaN3, 0.2% BSA. Isotype. Rabbit IgGk. Preparation. Protein A+G. Recommended Usage. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume.

mTOR-Dependent Autophagy Machinery Is Inhibited in Fibroblas

WebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, … WebRayBio® Human and Mouse Phospho-mTOR (Ser2448) and Total mTOR ELISA Kit. This assay semi-quantitatively measures mTOR phosphorylated at Serine-2448 as well as total mTOR in cell lysate samples. $567.00 CODE: PEL-mTOR-S2448-T-1 Number of Plates: Quantity: + − Add To Cart View all product options Description Reviews Ask a question … dynafil 100mg price south africa

Considerations on mTOR regulation at serine 2448: …

WebPhospho-mTOR (Ser2448) Antibody (46-9718-42) in Flow TOP: Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 Functional Grade … WebSer2448 phosphorylation is measured to assess mTOR kinase activation or as a measurement of induction of the mTOR pathway in muscle tissue and is implicated in mTORC1 activity [34,37,38,39]. The effects of SLC38A9 and SLC36A1 on mTOR phosphorylation at Ser2448 in C2C12 cells remains unclear. WebThe mTOR subunit of mTORC1 and 2 is phosphorylated at Ser2448 in a PI3/Akt- signaling-dependent manner; this post-translational modification is associated with increased … crystal springs foundation

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WebInvitrogen Anti-Phospho-mTOR (Ser2448) Polyclonal, Catalog # 44-1125G. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts … WebmTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by … crystal springs food and wineWebProduct Description. The PathScan ® Phospho-mTOR (Ser2448) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects … dyna firewood processor forum

"WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with … " - Phospho mtor ser2448

Phospho mtor ser2448

WebPhospho-mTOR (Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Serine 2448. RRID: AB_2834727 Cite Format: Affinity Biosciences Cat# AF3308, RRID:AB_2834727. Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho … WebWe show that Ser-2448 is directly phosphorylated byp70S6 kinase in vitro and that expression of a rapamycin-resistantp70S6 kinase in cells maintains Ser-2448 phosphorylation in the presence ofrapamycin. Furthermore, changes in amino acid availability, which affectsp70S6 kinase activity, also modulates Ser-2448 phosphorylation.

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WebInvitrogen Anti-Phospho-mTOR (Ser2448) Monoclonal (MRRBY), eBioscience™, Catalog # 12-9718-42. Tested in Flow Cytometry (Flow) applications. This antibody reacts with … WebPrimary antibodies for immunohistochemistry (IHC) included anti-mTOR, anti-phospho-mTOR Ser2448, anti-S6 ribosomal protein, anti-Beclin1 (all from Abcam, Cambridge, UK) …

WebJul 8, 2005 · Previous studies have shown that mTOR is a direct substrate for the AKT kinase and identified Ser-2448 as the AKT target site in mTOR. In this study, we … WebIn conclusion, it is recommended that Ser2448 is an inadequate measure and that preferential analysis of mTORC1 activation should focus on the downstream and effector …

WebJan 15, 2009 · mTOR phosphorylated at S2448 can be found in a complex with both, raptor and rictor. Total lysates of logarithmically growing HEK293 cells were prepared and used for immunoprecipitation of endogenous raptor or rictor. WebAug 24, 2005 · Anti-mTOR (phospho S2448) antibody [EPR426 (2)] (ab109268) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by …

WebmTOR抑制剂的筛选方法. 分析：OD值越小，mTOR的Ser2448磷酸化程度越低，抑制剂作用越强。. （2）荧光共振能量转移（FRET）. 荧光共振能量转移是指两个荧光发色基团在足够靠近（7~10nm）时，当供体分子吸收一定频率的光子后被激发到更高的电子能态，在该电子回 …

Webresults in elevated mTOR activity in vitro and in cells (18, 20). In this study, we demonstrate that mTOR phosphorylation at Ser-2448 is blocked by rapamycin, and this effect is inde-pendent of the AKT activation status, which suggests that Ser-2448 phosphorylation is catalyzed by a protein kinase other than AKT. We show that Ser-2448 is ... crystal springs ford dealershipWebThe Phospho-mTor (Ser2448) assay measures mTor when phosphorylated at Ser2448. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-mTor (Ser2448) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. crystal springs for businessWebmTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GTX132803 WB Image Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody … dyna firewood processor rental priceWebThe MSD Phospho(Ser2448)/Total mTOR Assay is available on 96-well 4-Spot plates. This datasheet outlines the performance of the assay. Typical Data Representative results for … dyna fat bob specsWebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, … dyna fire protectionWebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 213 publications. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and … crystal springs goat yogaWebPhospho-mTOR (Ser2448) (49F9) Rabbit mAb (BSA and Azide Free) detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human … dynafit 24/7 polartec zip hoody - beet red